Low power blue LED exposure increases effects of doxorubicin on MDA-MB-231 breast cancer cells.

Patients with triple negative breast cancer can develop side effects as a result of chemotherapy. Photodynamic therapy may reduce these side effects if the chemotherapy agent could also act as a photosensitizer. Thus, the aim of this work was to evaluate cytotoxicity and reactive oxygen species production induced by doxorubicin and low power blue LED in breast cancer cultures. Cell viability and reactive oxygen species (ROS) in MDA-MB-231 cultures were evaluated in response to different doxorubicin concentrations and blue LED fluences. Compared with control, cell cultures only incubated with doxorubicin at 25 nM showed 23% of cell viability reduction while its combination with blue LED at 640 J/cm2 reduced 40% of cell viability after 24 h. After 48 h, reduction of cell viability raises to 40% in cell cultures only incubated with doxorubicin and 55% when combined with blue LED. Evaluation 30 min after treatment showed that cells incubated with doxorubicin and exposed to blue LED generated 22% more ROS than controls. Those results show that incubation with doxorubicin combined with exposure to low power blue LED is more cytotoxic and more effective to increase ROS levels in MDA-MB-231 cultures than incubation with doxorubicin alone.

Polaciúria incapacitante para a vida usual. Relato de tuberculose da bexiga. A tuberculose permanece no rol do diagnóstico diferencial da maioria das doenças / Incapacitating pollakiuria affecting daily life. A case report of tuberculosis of the bladder. Tuberculosis remains on the list of the differential diagnoses for most diseases

A tuberculose (TB) é dos mais graves problemas de saúde humano e associa-se a pobreza, incipiência do sistema de saúde, deficiência de gestão que dificulta a diminuição de doenças de determinismo social, epidemia do vírus da imunodeficiência humana e imunodeficiência adquirida e multirresistência do M. tuberculosis. O acometimento extrapulmonar ocorre em 15% dos casos de TB e pode evoluir com amesma sintomatologia geral do acometimento pulmonar, com variações na dependência de sua localização e gravidade. Este relato apresenta paciente com hematúria e polaciúria incapacitante para a vida social, associada inicialmente à neoplasia, e determinada pela TB geniturinária, com diagnóstico após histopatologia de espécime clínico obtido da bexiga, em que o tempo longo para o diagnóstico foi determinante para grave sequela. Objetiva alertar para a TB como doença permanente no Brasil ea necessidade de ser considerada sempre como diagnóstico diferencial de doenças pulmonares ou extrapulmonares.

Tuberculosis (TB) is among the most serious human health concerns and is associated with poverty, limited availability of health care services and bad management, all of which negatively affect the possibility of reducing socially-determined diseases, such as HIV/AIDS epidemics and multidrug resistant M. tuberculosis. Extrapulmonary involvement occurs in 15% of TB cases and can develop with the same overall symptomatology of exclusively pulmonary involvement, with smaller variations depending on location and severity. This report presents a patient with hematuria and pollakiuria with incapacitating effects on social life, initially thought of as a malignancy and later identified as genitourinary TB. Diagnosis was based on histopathology of clinical specimens from the bladder. Time elapsed between onset and diagnosis led to considerable sequelae. This report aims at raising awareness of TB as a permanent concern in Brazil and of the need to always considered it as a differential diagnosis of pulmonary or extrapulmonary disease.

Incentivo à adoção de modos saudáveis de vida entre profissionais de saúde: Relato de uma intervenção na atenção primária / Encouraging the adoption of healthy ways of life among health workers: report of an intervention in primary care

Objetivou-se incentivar os funcionários de uma Unidade Básica de Saúde (UBS) para a adoção de modos saudáveis de vida. Trata-se de um relato de experiência, desenvolvida em uma UBS de Belo Horizonte/MG, com duração de quatro meses, abrangendo três etapas: 1. sensibilização dos profissionais quanto à importância da temática em foco e avaliação antropométrica dos participantes; 2. caracterização do consumo alimentar por meio de questionário quanti-qualitativo; e 3. ações de incentivo à adoção de modos saudáveis de vida, com intervenções diversificadas e duração de sete semanas. A experiência conduzida alcançou impacto positivo ao despertar para a mudança de modos de vida e denota importância de ações permanentes com profissionais no âmbito da Atenção Primária à Saúde. .

The objective was to encourage employees of a Basic Health Unit (BHU) to adopt healthier approaches in their daily lives. This article is an experience report developed at a BHU in Belo Horizonte / MG, over a 4 month period, covering three steps: 1. Raising awareness among these professionals about the importance of the topic in focus, and an anthropometric assessment of the participants; 2. Characterization of food intake using a quantitative and qualitative questionnaire; 3. Actions to encourage the adoption of healthier lifestyles, using diverse interventions, over a seven week period. This experience achieved a positive effect by creating changes to individuals? lifestyles, and indicates the importance of such actions, on a continuous basis, for professionals within the Primary Health Care system.

Processamento de máscaras para aerossóis: comparação entre os sistemas centralizado e descentralizado / Aerosol masks submitted to centralized and descentralized disinfection process

O objetivo do trabalho foi comparar os resultados do processamento de máscaras para aerossóis, pelos sistemas centralizado e descentralizado de desinfecção...